What is an Illumina adapter sequence?

These sequences are provided for the sole purpose of understanding and publishing the results of your sequencing experiments. Your permission to copy or distribute sequence information is limited to within your institution for use only with Illumina instruments and associated equipment, consumables, and software.

Are Illumina adapters methylated?

The sequences of NEBNext Methylated Adaptor and NEBNext Adaptor for Illumina are identical except that all cytosines in NEBNext adaptor are replaced with methylated cytosines.

How long are the Illumina adapters?

Illumina libraries are normally constructed by ligating adapters to short fragments (100 – 1000bp) of DNA.

Can Illumina detect DNA modifications?

Analyzing Epigenetic Alterations Illumina offers a broad portfolio of next-generation sequencing (NGS)- and array-based epigenetic analysis tools that provide robust, simple-to-use, and cost-efficient solutions for studying these epigenetic modifications and their impact on gene regulation.

Can Illumina sequencing detect methylation?

Two main types of technologies used to detect methylation signals are methylation arrays and sequencing [6]. Before the era of high-throughput sequencing, methylation arrays, such as Illumina Infinium, were widely used to detect methylation signals [2, 3].

Are adapters primers?

The adapters contain the sequencing primer binding sites, the index sequences, and the sites that allow library fragments to attach to the flow cell lawn. Because the sequencing starts at the first base of the DNA insert in Reads 1 and 2, the adapter is not sequenced at the start of the read.

What causes adapter dimers?

Adapter dimers are formed during the ligation portion of the protocol when two adapters are joined together. These are problematic because they can bind to the flow cell and undergo sequencing, but provide no data other than the sequence of the adapter present.

What is Illumina known for?

Illumina is a leading developer, manufacturer, and marketer of life science tools and integrated systems for large-scale analysis of genetic variation and function. These systems are enabling studies that were not even imaginable just a few years ago, and moving us closer to the realization of personalized medicine.

What do nebnext multiplex oligos do for Illumina?

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.

What do you need to know about Illumina adapters?

Our leaders are pioneers of genetic research and clinical applications. They are deeply committed to scientific accessibility, data, and community. Oligonucleotide (oligo) sequences of Illumina adapters used in library prep kits. This information is provided for use with Illumina instruments only.

How are oligonucleotide sequences used in Illumina prep kits?

They are deeply committed to scientific accessibility, data, and community. Oligonucleotide (oligo) sequences of Illumina adapters used in library prep kits. This information is provided for use with Illumina instruments only. Get instructions for sharing your desktop while working with Technical Support.

How are adapter sequences used in Illumina sequencing?

Illumina Adapter Sequences. This document provides the nucleotide sequences that comprise Illumina oligonucleotides used in Illumina sequencing technologies. These sequences are provided for the sole purpose of understanding and publishing the results of your sequencing experiments. Proprietary to Illumina .