What is a UV Stratalinker?

The Stratalinker® UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours.

How to use a UV crosslinker?

The UV Crosslinker can be operated on the following settings: A. Preset UV Energy Exposure Setting: Push the PRESET and then ENERGY on the tactile touch pad. The red light at each position should now be lit and the preprogrammed UV exposure setting of 120,000 microjoules per cm² is displayed in the LCD as 1200.

What type of irradiation does the Stratalinker deliver?

UV irradiation
UV irradiation (254 nm) was delivered from a Stratalinker by using mercury vapor bulbs and attenuated at all times with a neutral density filter (5% transmittance).

What does a crosslinker do?

In the standard definition, the crosslinker is an additive which links two polymer chains by the covalent or ionic bond. In polymer chemistry, the curing reaction changes mechanical properties or viscosity by increasing molecular weight of a polymer by reacting two different components.

What is the consequence to continual exposure to UV light in single celled organisms?

UV-B exposure can significantly affect phytoplankton, the single-celled organisms at the base of the marine food web. Excessive UV-B radiation can impair photosynthesis, inhibit phytoplankton growth rates, and cause lethal DNA damage.

What is a crosslinking agent?

Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable or chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. This article describes the chemistry and variety of crosslinkers that exist.

Why does rubber have to be cross linked to be useful?

Because they are tied together, when the rubber gets hot, they can’t flow past each other, nor around each other. This is why it doesn’t melt. Also, because all the polymer molecules are tied together, they aren’t easily broken apart from each other.

What is the effect of UV light on bacteria?

When UV light is in operation of DNA viruses, bacteria and other pathogens impacted. This will reduce the ability of the DNA to multiply and cause disease. Furthermore, the UV light destroys harmful microbes such as bacteria, yeast, moulds, viruses and algae.

What happens during crosslinking?

The procedure causes new corneal collagen cross-links to develop. Those cross links cause the collagen fibrils to shorten and thicken, leading to a stiffer, stronger cornea. Corneal cross linking is an outpatient procedure that typically lasts for about an hour.

Why rubber is vulcanised?

Vulcanization is a chemical process in which the rubber is heated with sulphur, accelerator and activator at 140–160°C. The process involves the formation of cross-links between long rubber molecules so as to achieve improved elasticity, resilience, tensile strength, viscosity, hardness and weather resistance.

How does the Stratagene UV crosslinker 1800 work?

Stratagene Stratalinker 1800. The Stratalinker® UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours.

How long does it take for a stratalinker to crosslink DNA?

The Stratalinker® UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours.

What do you need to know about Stratagene stratalinker?

Stratagene Stratalinker 1800 1 Used in Northern, Southern, dot or slot blot analysis and more 2 Autocrosslink, Time, and Energy modes 3 1800 UV ideal for smaller membranes More

How long does it take to crosslink a filter?

The crosslinking process takes only 25-50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking.